Randomized trial evaluates structural and functional differences in hiPSC-derived cardiomyocytes, suggesting improved methods for cardiac tissue engineering.
Key Points
This research aims to compare the structural and functional characteristics of hiPSC-derived cardiomyocytes generated through two different differentiation methods.
Evaluated hiPSC-CMs generated by two-dimensional monolayer (2D-Mono) and three-dimensional embryoid-body (3D-EB) differentiation.
Used identical 15-day Wnt-modulated protocols for both methods without additional maturation steps.
Measured cell yield, structural parameters, and functional performance metrics such as calcium conduction and contractility.
3D-EB method yielded approximately 181 × 10^6 cells per 100 ml, 2.7-fold higher than 2D-Mono with >80% cTnT+ purity.
3D-EB tissues showed improved sarcomeric organization and faster calcium conduction (33.7 cm/s) than 2D-Mono.
Despite similarities in contractile performance, 3D-EB exhibited consistent structural and functional improvements over time.