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July 12, 2026Lab AnimalOpen Access

Establishment and functional analysis of an inducible Apoc2-knockout mouse model to investigate the role of Apoc2 in normal hematopoiesis

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Authors

XZXiaochen ZhangUniversity of Southern CaliforniaRCRitu ChandwaniUniversity of Southern CaliforniaMPMateusz PospiechUniversity of Southern California

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Implication

Randomized trial investigates Apoc2's effect on normal hematopoiesis in mice, suggesting limited impact under standard conditions.

Key Points

  • This research aims to explore the role of Apoc2 in normal hematopoiesis using an inducible knockout mouse model.
  • Generated Apoc2 knockout mice by breeding R26-CreERT2 and Apoc2 fl/fl mice for three generations.
  • Induced global Apoc2 knockout in 4–8-week-old mice using tamoxifen for five consecutive days.
  • Conducted RNA-sequencing and analyzed hematological parameters in comparison to wild type mice.
  • Confirmed Apoc2 knockout with 80–99% reduction in mRNA levels across various tissues.
  • No significant differences in hematopoietic stem and progenitor cell expansion or colony-forming ability between knockout and wild type.
  • Identified four downregulated and five upregulated genes in the knockout group, with enrichment in heme metabolism.

Cite This Study

Zhang et al. (2026) studied this question.

synapsesocial.com/papers/6a5332484f7abc118adedd4dhttps://doi.org/10.1038/s41684-026-01748-z
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