What question did this study set out to answer?

To develop a low-cost and rapid biosensor for urea detection using immobilized urease from Canavalia ensiformis.

Canavalia ensiformis urease immobilization for urea biosensing: Rapid urea detection dipstick

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Randomized trial demonstrates a colorimetric biosensor's effectiveness in detecting urea, suggesting promising clinical applications.

To develop a low-cost and rapid biosensor for urea detection using immobilized urease from Canavalia ensiformis.
Used DEAE-cellulose membrane for enzyme immobilization.
Enzyme purified via ammonium sulfate precipitation and anion exchange chromatography.
Biosensor tested in artificial serum samples with triplicates (n = 3).
Biosensor had a limit of detection of 0.5 mM and a linear range of 0.5–12.5 mM.
Achieved 92–95% recovery compared to a spectrophotometric reference method.
Sensor stability maintained for up to six months at 4 °C.

Hussain et al. (2026) studied this question.

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View Full Paper

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Authors

Randomized trial demonstrates a colorimetric biosensor's effectiveness in detecting urea, suggesting promising clinical applications.

To develop a low-cost and rapid biosensor for urea detection using immobilized urease from Canavalia ensiformis.
Used DEAE-cellulose membrane for enzyme immobilization.
Enzyme purified via ammonium sulfate precipitation and anion exchange chromatography.
Biosensor tested in artificial serum samples with triplicates (n = 3).
Biosensor had a limit of detection of 0.5 mM and a linear range of 0.5–12.5 mM.
Achieved 92–95% recovery compared to a spectrophotometric reference method.
Sensor stability maintained for up to six months at 4 °C.

Hussain et al. (2026) studied this question.

View Full Paper

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Bookmark