Key result
Hypothermic storage of canine cardiac myocytes in University of Wisconsin solution preserved the beta-adrenergic response of Ca2+ channels (165% of controls) better than Stanford (130%) or St Thomas' (135%) solutions at 24 hours.
Why the study?
Does hypothermic storage in University of Wisconsin solution preserve the beta-adrenergic response of isolated canine ventricular myocytes compared to Stanford or St Thomas' solutions?
Does hypothermic storage in University of Wisconsin solution preserve the beta-adrenergic response of isolated canine ventricular myocytes compared to Stanford or St Thomas' solutions?
Absolute Event Rate: 165% vs 130%
Hypothermic storage of canine cardiac myocytes in University of Wisconsin solution better preserves the beta-adrenergic response of calcium channels compared to standard solutions.
May favor UW for experimental myocyte preservation; leaves open translation to clinical cardiac storage.
BACKGROUND: University of Wisconsin (UW) solution has been shown to be an effective solution for cold storage of various organs. This study was designed to evaluate the subcellular protective mechanism of UW solution during cardiac myocyte storage using patch-clamp techniques for the first time as a tool for the detection of myocyte viability. METHODS AND RESULTS: The protective effects of UW solution on the preservation of dihydropyridine-sensitive Ca2+ channel current response to catecholamine were evaluated in canine cardiac ventricular cells by measurement of single channel open probability. Single ventricular myocytes were isolated and stored in UW solution, in Stanford (SF) solution, or in St Thomas' (ST) solution at 4 degrees C for 2, 6, 12, and 24 hours, and after each storage period, recordings were made of cell-attached single Ca2+ channel currents. When 0.1 mumol/L isoproterenol was applied, percent mean open probability of the Ca2+ channel tested in freshly isolated cells was 167 +/- 4% (n = 24) of controls (100%). The response was decrescent with increased duration of the hypothermic storage and was only 130 +/- 12% (n = 4) after 24 hours of storage in SF solution and 135 +/- 9% (n = 7) in ST solution. However, it was significantly highly preserved as much as 165 +/- 9% (n = 6) in UW solution. Ca2+ channel kinetics and channel conductance were not changed after up to 24 hours of hypothermic storage. CONCLUSIONS: Hypothermic storage of canine cardiac myocytes in UW solution preserved beta-adrenergic response, which suggests that UW solution during cold storage preserved high-energy phosphates in myocytes that are responsible for Ca2+ channel phosphorylations.
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K et al. (1995) studied Isolated canine ventricular myocytes. University of Wisconsin (UW) solution vs. Stanford (SF) solution or St Thomas' (ST) solution was evaluated on Percent mean open probability of the Ca2+ channel in response to 0.1 mumol/L isoproterenol after 24 hours of storage. Hypothermic storage of canine cardiac myocytes in University of Wisconsin solution preserved the beta-adrenergic response of Ca2+ channels (165% of controls) better than Stanford (130%) or St Thomas' (135%) solutions at 24 hours.
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