Synapse
⌘+K
Synapse
PulseExploreClubsResearchersJournals
Instagram
HomeClubsExplore
July 20, 2026Analytical Chemistry

Tandem MICROFASP Method-Enabled High-Sensitivity In Vivo Cross-Linking Mass Spectrometry Analysis of Low-Cell-Number Samples without Enrichment

View Full Paper
Ask AI
Bookmark
Share

Authors

YHYu HeYLYang LiRLRong Liu

Discussion

Loading...

Member takes

Overview

Randomized trial demonstrates enhanced protein analysis in low-cell-number samples, indicating broader application potential.

Key Points

  • This research aims to improve the sensitivity and applicability of cross-linking mass spectrometry in low-cell-number samples.
  • Developed a tandem MICROFASP platform to integrate in vivo chemical cross-linking with in situ sample preparation.
  • Optimized experimental parameters including cross-linking duration, lysis buffer, and mass spectrometry acquisition.
  • Identified cross-linked peptides from K562 cells without pre-enrichment.
  • Identified 517 intercross-linked peptides from 10,000 K562 cells, a 3.5-fold increase compared to previous results.
  • Identified 318 cross-linked peptides from just 2500 living K562 cells.
  • Over 97% of identified cross-linked sites adhered to distance constraints of the cross-linker.

Cite This Study

He et al. (2026) studied this question.

synapsesocial.com/papers/6a5dba128bd453d3397ab5achttps://doi.org/10.1021/acs.analchem.6c01538
View Full Paper
Ask AI
Bookmark
Share