Effects of carotenoids fromDeinococcus radioduranson protein oxidation

AIMS: To evaluate the antioxidant effect of carotenoids from Deinococcus radiodurans on protein. METHODS AND RESULTS: Deinococcus radiodurans strain R1 (ATCC 13939) and its mutant strain R1DeltacrtB were used for this study. The total carotenoids (R1ex) from D. radiodurans were obtained by extraction with acetone/methanol (7 : 2, by vol), and their antioxidant activity was measured using the DPPH (2,2-diphenyl-1-picrylhydrazyl) system. The protein oxidation level, in vitro and in the cell, was measured using the DNPH (2,4-dinitrophenyl hydrazine) method. The carotenoid extract R1ex scavenged 40.2% DPPH radicals compared to beta-carotene (31.7%) at a concentration of 0.5 mg ml(-1). The intracellular level of protein oxidation in mutant R1DeltacrtB, which does not contain carotenoid, was 0.0212 mmol mg(-1) protein which is significantly greater than that in the wild type (0.0169 mmol mg(-1) protein) following the treatment with H(2)O(2). The purified major carotenoid product (deinoxanthin) from the wild type showed a greater inhibition of oxidative damage in bovine serum albumin than lycopene or lutein. CONCLUSIONS: Carotenoids prevent protein oxidation and contribute to the resistance to cell damage in D. radiodurans. SIGNIFICANCE AND IMPACT OF THE STUDY: Our results provide the evidence that carotenoids can protect proteins in D. radiodurans against oxidative stress.