Kohn and Liversedge (3) observed that various animal tissues after incubation under aerobic conditions, produce compound(s) which give a color reaction when heated with thiobarbituric acid (TBA). Although they did not demonstrate the nature of the color producing compound(s), their research established the absorption spectrum of the color complex and certain chemical characteristics of the color reaction. Research by Bernheim et al.
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Patton et al. (1951) studied this question.