What is the clinical evidence from this study?

Study design: Other. Population: Aging (n=40). Intervention: Aging vs. Young rats (5 months old). Primary outcome: Hypotensive response to acetylcholine, bradykinin, and sodium nitroprusside, and expression/activity of NOS isoforms.

What question did this study set out to answer?

The study aimed to evaluate nitric oxide synthase expression and hypotensive responses in aging versus young rats.

August 10, 1998Circulation ResearchOpen Access

Expression of Constitutive and Inducible Nitric Oxide Synthases in the Vascular Wall of Young and Aging Rats

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Key result

Aging in rats was associated with an impaired endothelium-dependent hypotensive response, enhanced expression of eNOS and iNOS isoforms, but reduced eNOS activity in the vessel wall.

Population

40 euvolemic, conscious, male Wistar rats (n=20 aging rats, 18 months old; n=20 young rats, 5 months old)

Comparison

Aging (18 months old) vs Young (5 months old)

Design

Preclinical

Authors

MCM.R. CernadasHospital Universitario Fundación Jiménez Díaz LMLourdes Sánchez de MiguelUniversity of Freiburg MGMargarita Garcı́a-DuránInstitut Català de la Salut

Discussion

Member takes

Implication

Hypothesis-generating for age-related eNOS uncoupling; leaves open translation to human endothelial therapies.

Key Points

The study aimed to evaluate nitric oxide synthase expression and hypotensive responses in aging versus young rats.
Analyzed hypotensive responses to acetylcholine, bradykinin, and sodium nitroprusside in 40 rats (20 young, 20 aging).
Evaluated nitric oxide synthase isoform expression via Western blot and assessed enzyme activity using [14C]L-arginine conversion.
Aging rats exhibited impaired hypotensive responses to acetylcholine and bradykinin, but maintained the response to sodium nitroprusside.
In aging rats, eNOS activity was significantly reduced despite increased expression of both eNOS and iNOS isoforms.
Plasma levels of nitrite and nitrate, as well as cGMP content, were elevated in aging rats.

Structured PICO

Population

40 euvolemic, conscious, male Wistar rats (20 aged 18 months, 20 aged 5 months) studied for endothelium-dependent and independent hypotensive responses.

Exposure

Aging (18 months old)

Comparator

Young (5 months old)

Outcome

Hypotensive response to acetylcholine, bradykinin, and sodium nitroprusside, and expression and activity of eNOS and iNOS isoformssurrogate

Aging in rats is associated with reduced eNOS activity despite increased eNOS and iNOS expression, explaining the impaired endothelium-dependent hypotensive response.

Cite This Study

Cernadas et al. (1998) studied Aging (n=40). Aging vs. Young rats (5 months old) was evaluated on Hypotensive response to acetylcholine, bradykinin, and sodium nitroprusside, and expression/activity of NOS isoforms. Aging in rats was associated with an impaired endothelium-dependent hypotensive response, enhanced expression of eNOS and iNOS isoforms, but reduced eNOS activity in the vessel wall.

synapsesocial.com/papers/6a5f1d2c4a32e80e4258fa9d https://doi.org/10.1161/01.res.83.3.279

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Expression of Constitutive and Inducible Nitric Oxide Synthases in the Vascular Wall of Young and Aging Rats

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Expression of Constitutive and Inducible Nitric Oxide Synthases in the Vascular Wall of Young and Aging Rats

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Also Consider

Also Consider