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Engineered Lactococcus lactis demonstrates enhanced diacetyl production through metabolic modifications.
We report the engineering of Lactococcus lactis for the efficient conversion of sugar into diacetyl by combining NADH-oxidase overproduction and alpha-acetolactate decarboxylase inactivation. Eighty percent of the carbon flux was found to be rerouted via alpha-acetolactate to the production of diacetyl by preloading the cells with NADH-oxidase before their use as a cell factory.
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Hugenholtz et al. (2000) studied this question.
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