Key result
Exogenously added Gs or its alpha subunit directly stimulated skeletal muscle T-tubule Ca2+ channels by factors of 2-3 in the presence of Bay K 8644 and 10-20 in its absence.
Population
Skeletal muscle T-tubule membranes incorporated into planar lipid bilayers
Comparison
Exogenously added human erythrocyte GTP gamma… vs Baseline activity (absence of Gs)
Design
Preclinical
Authors
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Gs stabilizes Ca2+ channels in excised patches and bilayers; leaves open physiological relevance in intact myocytes.
Effect estimate: stimulation by factors of 2-3 to 10-20
The stimulatory G protein Gs directly stimulates dihydropyridine-sensitive Ca2+ channels independent of cAMP-dependent protein kinase phosphorylation, demonstrating that a single G protein can regulate multiple effector systems.
Yatani et al. (1988) studied this question. Gs (stimulatory G protein of adenylyl cyclase) or its alpha subunit vs. absence of Gs was evaluated on T-tubule Ca2+ channel activity (stimulation by factors of 2-3 to 10-20). Exogenously added Gs or its alpha subunit directly stimulated skeletal muscle T-tubule Ca2+ channels by factors of 2-3 in the presence of Bay K 8644 and 10-20 in its absence.
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