Exogenously added Gs or its alpha subunit directly stimulated skeletal muscle T-tubule Ca2+ channels by factors of 2-3 in the presence of Bay K 8644 and 10-20 in its absence.
The stimulatory G protein Gs directly stimulates dihydropyridine-sensitive Ca2+ channels independent of cAMP-dependent protein kinase phosphorylation, demonstrating that a single G protein can regulate multiple effector systems.
Effect estimate: stimulation by factors of 2-3 to 10-20
We demonstrated recently that purified preparations of Gs, the stimulatory G protein of adenylyl cyclase, can stabilize Ca2+ channels in inside-out cardiac ventricle membrane patches stimulated prior to excision by the beta-adrenergic agonist isoprenaline or by the dihydropyridine agonist Bay K 8644 and that such preparations of Gs can restore activity to spontaneously inactivated cardiac Ca2+ channels incorporated into planar lipid bilayers (Yatani, A., Codina, J., Reeves, J.P., Birnbaumer, L., and Brown, A.M. (1987) Science 238, 1288-1292). To test whether these effects represented true stimulation and to further identify the G protein responsible, we incorporated skeletal muscle T-tubule membranes into lipid bilayers and studied the response of their Ca2+ channels to G proteins, specifically Gs, and manipulations known to be specific for Gs. In contrast to cardiac channels, incorporated T-tubule Ca2+ channels exhibit stable average activities over prolonged periods of time (up to 20 min at room temperature), allowing assessment of possible effects of G proteins under steady-state assay conditions. We report that exogenously added human erythrocyte GTP gamma S (guanosine 5'-O-(3-thiotriphosphate]-activated Gs (Gs) or its resolved GTP gamma S-activated alpha subunit (alpha s) stimulate T-tubule Ca2+ channels by factors of 2-3 in the presence of Bay K 8644, and of 10-20 in the absence of Bay K 8644 and that they do so in a manner that is independent of concurrent or previous phosphorylation by cAMP-dependent protein kinase. Activation of purified Gs by cholera toxin increases both its adenylyl cyclase stimulatory and its Ca2+ channel stimulatory effects. Ca2+ channels previously stimulated by the combined actions of Bay K 8644 and cAMP-dependent protein kinase still respond to Gs. We conclude that the responses seen are due to Gs rather than a contaminant, that the effect on Ca2+ channel activity is that of a true stimulation, akin to that on adenylyl cyclase, and show that a given G protein may regulate more than one effector system.
Yatani et al. (Fri,) reported a other. Gs (stimulatory G protein of adenylyl cyclase) or its alpha subunit vs. absence of Gs was evaluated on T-tubule Ca2+ channel activity (stimulation by factors of 2-3 to 10-20). Exogenously added Gs or its alpha subunit directly stimulated skeletal muscle T-tubule Ca2+ channels by factors of 2-3 in the presence of Bay K 8644 and 10-20 in its absence.