Single-nucleotide polymorphism-based epidemiological analysis of KoreanMycobacterium bovisisolates

BACKGROUND: have not been performed in Korea. OBJECTIVES: groups in Korea by comparing SNP typing with spoligotyping and variable number tandem repeat typing. METHODS: field isolates, isolated from laryngopharyngeal lymph nodes and lungs of Korean cattle, wild boar, and Korean water deer, were used to identify SNPs by performing whole-genome sequencing. SNP sites were confirmed via polymerase chain reaction using 87 primer pairs. RESULTS: isolates, and performed SNP typing and epidemiological analysis, which divided the 46 field isolates into 16 subtypes. CONCLUSIONS: Through SNP analysis, detailed differences in samples with identical spoligotypes could be detected. SNP analysis is, therefore, a useful epidemiological tracing tool that could enable better management of bovine TB, thus preventing further outbreaks and reducing the impact of this disease.