Key result
7-substituted GDP analogs (7-methyl-, 7-ethyl-, and 7-benzyl-GDP) at 0.1 mM decreased stable initiation complex formation of reovirus mRNA to wheat germ ribosomes by 70 to 80%.
Population
Wheat germ ribosomes and reovirus mRNA
Comparison
Chemically synthesized compounds related to the… vs GDP and other cap analogs
Design
Preclinical
Authors
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May inform design of translation inhibitors; leaves open any clinical applications pending validation.
The positive charge resulting from 7-alkylation of the mRNA cap provides an active conformer for ribosomal binding.
Adams et al. (1978) studied this question. 5'-terminal "cap" analogs (e.g., 7-methyl-, 7-ethyl-, and 7-benzyl-GDP) vs. GDP was evaluated on Stable initiation complex formation (reovirus mRNA binding to wheat germ ribosomes). 7-substituted GDP analogs (7-methyl-, 7-ethyl-, and 7-benzyl-GDP) at 0.1 mM decreased stable initiation complex formation of reovirus mRNA to wheat germ ribosomes by 70 to 80%.
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