Reovirus mRNA can be covalently crosslinked via the 5' cap to proteins in initiation complexes.
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Population
Cell-free protein-synthesizing systems derived from wheat germ or mammalian cells and reovirus mRNA.
Design
Preclinical
Authors
Discussion
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Implication
No immediate clinical implications in cardiovascular care; leaves open the identity of cap-proximal initiation factors in human translation.
Structured PICO
P
Population
Cell-free protein-synthesizing systems derived from wheat germ or mammalian cells (rabbit reticulocyte lysate, mouse ascites ribosomes) and reovirus mRNA.
I
Intervention
Chemical crosslinking of oxidized reovirus mRNA to proteins in initiation complexes using NaBH3CN.
O
Outcome
Identification of covalently linked protein-RNA conjugates (polypeptides associated with mRNA-ribosome complexes).surrogate
Chemical crosslinking reveals specific proteins positioned near the 5' cap of mRNA during translation initiation in eukaryotic cells.
Cite This Study
Sonenberg et al. (1977) studied this question.
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