Capped mRNAs with Reduced Secondary Structure Can Function in Extracts from Poliovirus-Infected Cells
Population
Extracts from poliovirus-infected HeLa cells
Comparison
Denatured reovirus mRNAs and capped alfalfa… vs Native reovirus mRNA and other capped mRNAs
Design
Preclinical
Authors
Discussion
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Member takes
Implication
No immediate clinical implications; leaves open whether mRNA secondary structure selectively regulates translation in viral infection.
Structured PICO
P
Population
Extracts from poliovirus-infected HeLa cells
I
Intervention
Denatured reovirus mRNAs and capped alfalfa mosaic virus 4 RNA
C
Comparator
Native reovirus mRNA and other capped mRNAs
O
Outcome
Ribosome binding and translation efficiencysurrogate
Capped mRNAs with reduced secondary structure can function in extracts from poliovirus-infected cells, suggesting secondary structure limits translation in this context.
Cite This Study
Sonenberg et al. (1982) studied this question.