Molecular Diagnosis of Leishmaniasis in Spain: Development and Validation of Ready-To-Use Gel-Form Nested and Real-Time PCRs To Detect Leishmania spp.

Q: What does this research mean for the field?

Ready-to-use gel-form nested and real-time PCRs provide higher sensitivity than traditional methods for diagnosing leishmaniasis, facilitating early detection even in low-resource settings. Novelty: ClaimNovelty.METHODOLOGICAL. Consensus alignment: ConsensusAlignment.NEUTRAL.

Carmen Chicharro; Javier Nieto; Silvia Migueláñez; E. Barbero García; Sheila Ortega; Ana Peña; José Miguel Rubio; María Flóres-Chávez

doi:10.1128/spectrum.03354-22

April 4, 2023Microbiology SpectrumOpen Access

Molecular Diagnosis of Leishmaniasis in Spain: Development and Validation of Ready-To-Use Gel-Form Nested and Real-Time PCRs To Detect Leishmania spp.

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Key Points

To develop and validate ready-to-use gel-form nested and real-time PCR systems for detecting Leishmania spp. in clinical settings.
Developed a gel-form nested PCR and a real-time PCR system for Leishmania detection
Validated the sensitivity and specificity of these molecular diagnostic methods
Applicable for use in middle- and low-resource laboratories
Demonstrated higher sensitivity of molecular diagnosis compared to traditional methods
Facilitated early diagnosis leading to timely treatment of human leishmaniasis

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Implication

Shows higher sensitivity of molecular diagnosis for leishmaniasis, suggesting timely treatment in varied resource settings.

Abstract

spp. These new approaches could be introduced even in middle- and low-resource laboratories; one is a ready-to-use gel-form system of a nested PCR and the other is a real-time PCR. We show why molecular diagnosis is the best methodology to confirm a clinical suspicion of leishmaniasis with higher sensitivity than traditional methods, thus facilitating early diagnosis and timely treatment of human leishmaniasis.

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Chicharro et al. (2023) studied this question.

synapsesocial.com/papers/6a6220a0ce9bb37a6d7da954 https://doi.org/10.1128/spectrum.03354-22

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Molecular Diagnosis of Leishmaniasis in Spain: Development and Validation of Ready-To-Use Gel-Form Nested and Real-Time PCRs To Detect Leishmania spp.

Key Points

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Implication

Abstract

Discussion

Cite This Study

Molecular Diagnosis of Leishmaniasis in Spain: Development and Validation of Ready-To-Use Gel-Form Nested and Real-Time PCRs To Detect Leishmania spp.

Key Points

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Implication

Abstract

Discussion

Cite This Study

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