Key result
Implantation of a cellularized scaffold in rats post-myocardial infarction resulted in a comparable incidence of sustained ventricular tachyarrhythmias compared to saline controls.
Why the study?
Does a cellularized biomaterial scaffold implanted after myocardial infarction increase arrhythmogenic potential in a rat model?
Does a cellularized biomaterial scaffold implanted after myocardial infarction increase arrhythmogenic potential in a rat model?
Implantation of a cellularized scaffold in a rat myocardial infarction model showed low arrhythmogenic potential at two weeks, supporting its medium-term electrophysiologic safety.
Answers are grounded in this paper and cite their sources.
Supports preclinical safety of cellularized scaffolds post-MI; leaves open translation to clinical use and long-term effects.
Background Cardiac repair strategies are being evaluated for myocardial infarctions, but the safety issues regarding their arrhythmogenic potential remain unresolved. By utilizing the in-vivo rat model, we have examined the medium-term electrophysiologic effects of a biomaterial scaffold that has been cellularized with spheroids of human adipose tissue, derived from mesenchymal stem cells and umbilical vein endothelial cells. Methods Mesenchymal stem cells, which exhibit adequate differentiation capacity, were co-cultured with umbilical vein endothelial cells and were seeded on an alginate based scaffold. After in-vitro characterization, the cellularized scaffold was implanted in (n=15) adult Wistar rats 15 min post ligation of the left coronary artery, with an equal number of animals serving as controls. Two weeks thereafter, monophasic action potentials were recorded and activation-mapping was performed with a multi-electrode array. An arrhythmia score for inducible ventricular tachyarrhythmias was calculated after programmed electrical stimulation. Results The arrhythmia score was comparable between the treated animals and controls. No differences were detected in the local conduction at the infarct border and in the voltage rise in monophasic action potential recordings. Treatment did not affect the duration of local repolarization, but tended to enhance its dispersion. Conclusions The fabricated bi-culture cellularized scaffold displayed favorable properties after in-vitro characterization. Medium-term electrophysiologic assessment after implantation in the infarcted rat myocardium revealed low arrhythmogenic potential, but the long-term effects on repolarization dispersion will require further investigation.
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Kolettis et al. (2018) studied Myocardial Infarction (n=36). Alginate scaffold cellularized with hAT-MSCs and HUVECs vs. Normal saline intra-myocardial injection was evaluated on Incidence of sustained ventricular tachyarrhythmias. Implantation of a cellularized scaffold in rats post-myocardial infarction resulted in a comparable incidence of sustained ventricular tachyarrhythmias compared to saline controls.
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