Differential effect of pulsatile pressure on the myogenic responses of small arteries

Fig. 1. Effect of static and pulsatile pressure on the diameter of rat small tail arteries. A) Original recording of vessel diameter at a static pressure of 80 mmHg and in response to pulsatile pressure with different amplitudes (20, 40, and 60 mmHg) but unchanged mean pressure (80 mmHg). B) Diameter change (in % of initial diameter value) induced by static pressure at 80mmHg (con) and pulsatile pressure with different amplitudes (20, 40, and 60 mmHg) but unchanged mean pressure (80 mmHg). Repeated measures one-way ANOVA: n=6; p<0.001; multiple comparison with control (con) only by controlling for false discovery rate with two-stage linear step-up procedure of Benjamini, Krieger and Yekutieli: n=6; * - p=0.033, p<0.001, p<0.001, respectively. C) Diameter change (in % of initial diameter value) induced by a static pressure step (80 to 110 mmHg) and pulsatile pressure with different amplitudes (20, 40, and 60 mmHg) but unchanged systolic pressure (110 mmHg). Repeated measures one-way ANOVA: n=8; p=0.90

Fig. 2. Effect of static and pulsatile pressure on the diameter of rat small tail arteries. A) Normalized vessel diameter at different levels of transmural pressure for static pressure (con), pulsatile pressure (10 to 70, 50 to 110, and 90 to 150 mmHg) expressed as its mean pressure (osc), and for static pressure in calcium-free experimental solution (Ca2+-free). Repeated measures two-way ANOVA (effect of treatment): n=5; p<0.001; multiple comparison by controlling for false discovery rate with two-stage linear step-up procedure of Benjamini, Krieger and Yekutieli (con vs osc): n=5; * - p=0.004 (40 mmHg), p<0.001 (80 mmHg), p=0.02 (120 mmHg), respectively. B) Normalized vessel diameter at different levels of transmural pressure for static pressure (con), pulsatile pressure (10 to 70, 50 to 110, and 90 to 150 mmHg) expressed as its systolic pressure (osc), and for static pressure in calcium-free experimental solution (Ca2+-free). Repeated measures two-way ANOVA for linear regression parameters (con vs osc): n=5; p=0.38. C) Normalized vessel diameter at different levels of transmural pressure for static pressure (con), pulsatile pressure (20 to 40, 20 to 80 and 60 to 120 mmHg) expressed as…

Fig. 3. Effect of static and pulsatile pressure on tone, intracellular calcium and calcium sensitivity of rat small tail arteries. A) Vessel tone at different levels of transmural pressure for static pressure (con) and pulsatile pressure (10 to 70, 50 to 110, and 90 to 150 mmHg) expressed as its systolic pressure (osc). Repeated measures two-way ANOVA for linear regression parameters (con vs osc): n=5; p=0.90. B) Normalized fluorescence-ratio (F340/F380) at different levels of transmural pressure for static pressure (con) and pulsatile pressure (10 to 70, 50 to 110, and 90 to 150 mmHg) expressed as its systolic pressure (osc). Repeated measures two-way ANOVA for linear regression parameters (con vs osc): n=5; p=0.86. C) Relationship between the normalized fluorescence-ratio (F340/F380) and vessel tone for vessel responses induced by changes in static pressure (con) and pulsatile pressure (10 to 70, 50 to 110, and 90 to 150 mmHg) (osc). t-test for normalized fluorescence-ratio (F340/F380) at vessel tone of 25%: n=5; p=0.12

Fig. 4. Effect of static and pulsatile pressure on the diameter of rat gracilis arteries. A) Normalized vessel diameter at different levels of transmural pressure for static pressure (con), pulsatile pressure (10 to 70, 50 to 110, and 90 to 150 mmHg) expressed as its mean pressure (osc), and for static pressure in calcium-free experimental solution (Ca2+-free). Repeated measures two-way ANOVA (con vs osc): n=6; p=0.54. B) Normalized vessel diameter at different levels of transmural pressure for static pressure (con), pulsatile pressure (20 to 40, 20 to 60 and 60 to 120 mmHg) expressed as its mean pressure (osc), and for static pressure in calcium-free experimental solution (Ca2+-free). Repeated measures two-way ANOVA for linear regression parameters (con vs osc): n=9; p=0.60. C) Normalized vessel diameter at different levels of transmural pressure for static pressure (con), pulsatile pressure (20 to 40, 20 to 80 and 60 to 120 mmHg) expressed as its systolic pressure (osc), and for static pressure in calcium-free experimental solution (Ca2+-free). Repeated measures two-way ANOVA (effect of pressure): n=9; p<0.001; multiple comparison by controlling for false discovery rate with two-stage…

Fig. 5. Effect of static and pulsatile pressure on tone, intracellular calcium and calcium sensitivity of rat gracilis arteries. A) Vessel tone at different levels of transmural pressure for static pressure (con) and pulsatile pressure (10 to 70, 50 to 110, and 90 to 150 mmHg) expressed as its mean pressure (osc). Repeated measures two-way ANOVA (con vs osc): n=6; p=0.51. B) Normalized fluorescence-ratio (F340/F380) at different levels of transmural pressure for static pressure (con) and pulsatile pressure (10 to 70, 50 to 110, and 90 to 150 mmHg) expressed as its mean pressure (osc). Repeated measures two-way ANOVA (effect of pressure): n=6; p<0.001; multiple comparison by controlling for false discovery rate with two-stage linear step-up procedure of Benjamini, Krieger and Yekutieli (con vs osc): n=6; * - p=0.02 (40 mmHg), p=0.009 (80 mmHg), p=0.01 (120 mmHg), respectively. C) Normalized fluorescence-ratio (F340/F380) at different levels of transmural pressure for static pressure (con) and pulsatile pressure (10 to 70, 50 to 110, and 90 to 150 mmHg) expressed as its systolic pressure (osc). Repeated measures two-way ANOVA for linear regression parameters (con vs osc): n=5; p=0.47 D)…