Key result
Detubulation of rat ventricular trabeculae reduced twitch tension and slowed contraction kinetics, which was rescued by the RyR2 Ca-sensitizing agent caffeine (200 µM).
Population
Intact rat right ventricular trabeculae and left atrial trabeculae
Comparison
Formamide-induced osmotic shock and subsequent… vs Control trabeculae
Design
Preclinical
Authors
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T-tubule uncoupling effects on trabecular force and Ca2+ remain to be defined in disease; leaves open relevance to human HF mechanics.
Partial loss of t-tubules causes myocardial contractile abnormalities that can be rescued by enhancing the propagation of Ca(2+)-induced Ca(2+) release.
Ferrantini et al. (2014) studied this question. Detubulation (formamide-induced osmotic shock) vs. Control trabeculae was evaluated on Force, sarcomere length, and intracellular Ca(2+). Detubulation of rat ventricular trabeculae reduced twitch tension and slowed contraction kinetics, which was rescued by the RyR2 Ca-sensitizing agent caffeine (200 µM).
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