A simple isocratic h.p.l.c. technique was developed for the simultaneous quantitative analysis of lactic, formic, acetic, propionic, isobutyric, n ‐butyric and isovaleric acids in aqueous acid extracts of silage. An Aminex HPX‐87H strong cation exchange resin column at 41°C, 0.0025 M H 2 SO 4 mobile phase and ultraviolet detector at 210 nm were utilised. Estimated recoveries of acids added to silage ranged from 98.2 to 103.5%.
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Canale et al. (1984) studied this question.
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