This study was conducted to investigate the effects of different dietary protein and lipid levels on the growth performance, hepatic antioxidant capacity, and protein-lipid metabolism of juvenile Pelophylax nigromaculatus. A 4×2 factorial design was used, with dietary protein levels set at 38.5%, 41%, 43.5%, and 46%, and dietary lipid levels of 8% and 10%, resulting in the formulation of 8 experimental diets. Juvenile P.nigromaculatus with an initial body weight of (15.00±0.10) g were fed the diets for 8 weeks. The results indicated that the average weight gain rate (WGR) initially increased and then decreased with increasing dietary protein and lipid levels. The 41P10L group achieved the highest WGR, but no significant difference was observed compared with the 38.5P10L and 41P8L groups (P>0.05). Additionally, the 38.5P10L group showed the highest protein efficiency ratio (PER) (PPP.nigromaculatus was aggravated at the 10% lipid level, particularly under 43.5% and 46% protein levels (PPmtor and s6k in the 46% protein group were significantly higher than those in the 38.5% protein group (Pcpt1 expression level in the 10% lipid group was significantly higher than that in the 8% lipid group (PP.nigromaculatus was determined to be 38.5%, with an optimal dietary lipid level of 10%.
SHI et al. (Wed,) studied this question.