Key result
Elution with 3% beef extract followed by organic flocculation yielded a mean poliovirus recovery efficiency of 85%, compared to 36% with 0.1 M glycine and inorganic flocculation.
Population
20- to 25-gallon samples of treated sewage effluent seeded with Poliovirus type I, strain LSc
Comparison
Elution with two 600-ml portions of 3% beef… vs Elution with two 1-liter portions of 0.1 M…
Design
Other
Authors
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Beef extract elution may improve lab recovery; leaves open standardized field protocols for environmental virus monitoring.
Absolute Event Rate: 85% vs 36%
Beef extract elution with organic flocculation is superior to glycine elution with inorganic flocculation for recovering poliovirus from wastewater.
Landry et al. (1978) studied this question. 3% beef extract (pH 9.0) and organic flocculation vs. 0.1 M glycine (pH 11.5) and inorganic flocculation was evaluated on Mean recovery efficiency of poliovirus. Elution with 3% beef extract followed by organic flocculation yielded a mean poliovirus recovery efficiency of 85%, compared to 36% with 0.1 M glycine and inorganic flocculation.
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