Key result
The 0.5% isoelectric casein method shows no benefit over alternative methods for recovering soil poliovirus.
Why the study?
Efficient virus recovery methods from soil are needed to detect low levels of poliovirus and other enteroviruses in different soil types.
Population
Poliovirus type 1 and other enteroviruses in various Florida soils
Comparison
Isoelectric casein, non-fat dry milk, beef extract, and glycine-EDTA recovery methods
Design
Comparative evaluation of virus recovery methods
Authors
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Comparable soil poliovirus recovery supports 0.5% isoelectric casein use; leaves open optimal method standardization.
Bitton et al. (1979) studied this question. 0.5% (w/v) isoelectric casein at pH 9.0 vs. non-fat dry milk, beef extract, and glycine-EDTA methods was evaluated on Overall virus recovery. The 0.5% isoelectric casein method achieved 75% overall recovery of low input poliovirus from soil, with no significant difference in overall recovery compared to three other methods.
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