Key result
In human cardiac microvascular endothelial cells, H2O2-induced oxidant stress activates NOS3 via Akt, but Akt independently restores barrier integrity without NOS3/NO involvement.
Akt is critical for the restoration of barrier integrity in human cardiac microvascular endothelial cells following oxidant stress, independent of NOS3 regulation.
Akt may be a target for endothelial barrier protection independent of NO; leaves open translation from cell models to clinical microvascular disease.
The integrity of microvascular endothelium is an important regulator of myocardial contractility. Microvascular barrier integrity could be altered by increased reactive oxygen species (ROS) stress seen within minutes after cardiac arrest resuscitation. Akt and its downstream target nitric oxide (NO) synthase (NOS)3 can protect barrier integrity during ROS stress, but little work has studied these oxidant stress responses in human cardiac microvascular endothelial cells (HCMVEC). We, therefore, studied how ROS affects barrier function and NO generation via Akt and its downstream target NOS3 in HCMVEC. HCMVEC exposed to 500 microM H2O2 had increased Akt phosphorylation within 10 min at both Ser-473 and Thr-308 sites, an effect blocked by the phosphatidylinositol 3-kinase inhibitor LY-294002. H2O2 also induced NO generation that was associated with NOS3 Ser-1177 site phosphorylation and Thr-495 dephosphorylation, with Ser-1177 effects attenuated by LY-294002 and an Akt inhibitor, Akt/PKB signaling inhibitor-2 (API-2). H2O2 induced significant barrier disruption in HCMVEC within minutes, but recovery started within 30 min and normalized over hours. The NOS inhibitor Nomega-nitro-L-arginine methyl ester (200 microM) blocked NO generation but had no effect on H2O2-induced barrier permeability or the recovery of barrier integrity. By contrast, the Akt inhibitor API-2 abrogated HCMVEC barrier restoration. These results suggest that oxidant stress in HCMVEC activates NOS3 via Akt. NOS3/NO are not involved in the regulation of H2O2-affected barrier function in HCMVEC. Independent of NOS3 regulation, Akt proves to be critical for the restoration of barrier integrity in HCMVEC.
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Dossumbekova et al. (2008) studied Oxidant stress. H2O2 and Akt/NOS inhibitors was evaluated on Barrier function and NO generation. In human cardiac microvascular endothelial cells, H2O2-induced oxidant stress activates NOS3 via Akt, but Akt independently restores barrier integrity without NOS3/NO involvement.
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