Key result
Repeated injection of skeletal myoblasts in a swine model of chronic MI significantly improved cardiac function, with a correlation between total transplanted cells and LVEF improvement (P<0.05).
Why the study?
Does sequential transplantation of autologous skeletal myoblasts improve cardiac function in a swine model of chronic myocardial infarction?
Does sequential transplantation of autologous skeletal myoblasts improve cardiac function in a swine model of chronic myocardial infarction?
p-value: p=<0.05
Repeated percutaneous delivery of autologous skeletal myoblasts dose-dependently improves cardiac function, increases vasculogenesis, and decreases fibrosis in a swine model of chronic myocardial infarction.
Supports repeated percutaneous SkM delivery in chronic MI models; extends single-dose data toward optimized engraftment protocols.
AIMS: Although transplantation of skeletal myoblast (SkM) in models of chronic myocardial infarction (MI) induces an improvement in cardiac function, the limited engraftment remains a major limitation. We analyse in a pre-clinical model whether the sequential transplantation of autologous SkM by percutaneous delivery was associated with increased cell engraftment and functional benefit. METHODS AND RESULTS: Chronically infarcted Goettingen minipigs (n = 20) were divided in four groups that received either media control or one, two, or three doses of SkM (mean of 329.6 x 10(6) cells per dose) at intervals of 6 weeks and were followed for a total of 7 months. At the time of sacrifice, cardiac function was significantly better in animals treated with SkM in comparison with the control group. A significantly greater increase in the DeltaLVEF was detected in animals that received three doses vs. a single dose of SkM. A correlation between the total number of transplanted cells and the improvement in LVEF and DeltaLVEF was found (P < 0.05). Skeletal myoblast transplant was associated with an increase in tissue vasculogenesis and decreased fibrosis (collagen vascular fraction) and these effects were greater in animals receiving three doses of cells. CONCLUSION: Repeated injection of SkM in a model of chronic MI is feasible and safe and induces a significant improvement in cardiac function.
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Gavira et al. (2009) studied chronic myocardial infarction (n=20). Skeletal myoblast (SkM) transplantation vs. media control was evaluated on cardiac function (LVEF and DeltaLVEF) (p=<0.05). Repeated injection of skeletal myoblasts in a swine model of chronic MI significantly improved cardiac function, with a correlation between total transplanted cells and LVEF improvement (P<0.05).
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