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The technic involves the use of disposable micro tissue culture plates for either a simple screening test for lymphocyte competence or a complete and accurate morphologic and biochemical characterization of the transformation response. Because of the micro volumes used, the method is useful for transformation studies of lymphopenic patients and small children; for studies in which the effects of multiple concentrations of antigens, sera, mitogens, or chemicals are tested; and for performing the mixed lymphocyte reaction for histocompatibility studies. The simple screening test can be readily and effectively used in any clinical laboratory.
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Parker et al. (1971) studied this question.
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