Modulation of antiviral genes by African swine fever isolates of diverse virulence

Figures

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  Figure 1. Intracellular levels of early and late viral protein in ASFV-infected moMΦ 21 h post-infection (hpi). Porcine moMΦ were infected with the attenuated NH/P68 or the virulent 26544/OG10, using a MOI of 1, alongside mock-infected controls. 21 hpi, intracellular levels of the early protein p30 and the late protein p72 of ASFV were analyzed by flow cytometry. Data from four independent experiments utilizing different blood donors are presented. NH/P68 and 26544/OG10 data were compared using an unpaired t-test; ***p < 0.001, ****p < 0.0001.

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  Figure 2. Modulation of 84 antiviral genes in moMΦ 3 h post-infection (hpi). Porcine moMΦ were infected with the attenuated NH/P68 or the virulent 26544/OG10, using a MOI of 1, alongside mock-infected controls. 3 hpi, macrophages were analyzed using the RT2 Profiler PCR Array for 84 antiviral genes. (A) The heatmap illustrates fold change expression of immune-related genes. Data from four independent experiments utilizing different blood donors are presented. For each ASFV strain, fold change in gene expression relative to the mock-infected control was calculated, and colors represent these fold changes: yellow represents the highest value, white the baseline value (fold change = 1), and blue the smallest value. (B) Statistically significantly up-regulated (fold change > 2, p-value < 0.05) and down-regulated (fold change < 0.5, p-value < 0.05) genes are presented, with the corresponding fold change and p-value.

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  Figure 3. Modulation of 84 antiviral genes in moMΦ 21 h post-infection (hpi). Porcine moMΦ were infected with the attenuated NH/P68 or the virulent 26544/OG10, using a MOI of 1, alongside mock-infected controls. 21 hpi, macrophages were analyzed using the RT2 Profiler PCR Array for 84 antiviral genes. (A) The heatmap illustrates fold change expression of immune-related genes. Data from four independent experiments utilizing different blood donors are presented. For each ASFV strain, fold change in gene expression relative to the mock-infected control was calculated, and colors represent these fold changes: yellow represents the highest value, white the baseline value (fold change = 1), and blue the smallest value. (B) Statistically significantly up-regulated (fold change > 2, p-value < 0.05) and down-regulated (fold change < 0.5, p-value < 0.05) genes are presented, with the corresponding fold change and p-value.