Systemic treatment with superoxide scavengers Tempol or Tiron prevented the loss of microvessels in spontaneously hypertensive rats (411 and 399 mm/mm3 vs 371 mm/mm3 in untreated controls).
Does antioxidant treatment with superoxide scavengers prevent endothelial cell apoptosis and microvessel rarefaction in spontaneously hypertensive rats?
Antioxidant treatment with superoxide scavengers inhibits endothelial cell apoptosis and prevents microvessel rarefaction in spontaneously hypertensive rats.
Absolute Event Rate: 411% vs 371%
p-value: p=<0.01
OBJECTIVE: Endothelial cell apoptosis caused by oxidative stress may lead to the loss of microvessels (rarefaction) in hypertension. We examine here the effects of antioxidants on cell apoptosis and rarefaction. METHODS AND RESULTS: The juvenile spontaneously hypertensive rats (SHR) and normotensive Wistar-Kyoto (WKY) rats were treated with superoxide scavengers, Tempol or Tiron, during growth. After the treatment, oxidative stress status, endothelial cell apoptosis rate, and microvessel length density in skeletal muscle and mesentery were evaluated in comparison with age-matched controls. Untreated 16-week-old SHR had higher oxidative stress (P<0.01) and cell apoptosis rate (P<0.05) and lower microvessel length density (371+/-17 mm/mm3 P<0.01) compared with age-matched WKY rats (435+/-15 mm/mm3). In the SHR, but not in WKY rats, systemically applied antioxidants attenuated oxidative stress and cell apoptosis rate (P<0.05 versus untreated controls) and prevented the loss of microvessels (411+/-15 mm/mm3 for Tempol P<0.01 versus untreated control and 399+/-17 mm/mm3 for Tiron P<0.05). CONCLUSIONS: Antioxidant treatment with cell-permeable superoxide scavengers inhibits endothelial cell apoptosis and prevents microvessel rarefaction in the SHR during growth.
Kobayashi et al. (Fri,) conducted a other in Hypertension. Superoxide scavengers (Tempol or Tiron) vs. Untreated age-matched controls was evaluated on Microvessel length density (p=<0.01). Systemic treatment with superoxide scavengers Tempol or Tiron prevented the loss of microvessels in spontaneously hypertensive rats (411 and 399 mm/mm3 vs 371 mm/mm3 in untreated controls).