Key result
A simple ImageJ-based method using a dissecting microscope and CCD camera successfully measured cardiac rhythm and detected astemizole-induced heart arrhythmia in zebrafish embryos.
This study provides a simple, economical, and reliable open-source method to measure cardiac rhythm and detect arrhythmias in zebrafish embryos for chemical compound evaluation.
Simplifies zebrafish heart rate assessment with basic equipment; leaves open validation in mammalian models before research adoption.
Zebrafish has an advantage of optical clarity within the body during its early developmental stage. Therefore, organs, including the heart, can be observed and analyzed in a non-invasive manner. Previously, heart rate measurement was analyzed by a complicated system, programming language, fluorescence transgenic zebrafish, or expensive setup that was not feasible and practical. In this study, we established a simple dissecting microscope with Charge-coupled Device (CCD) to capture the signal of heartbeat, and used ImageJ software as an open source platform to analyze cardiac rhythm for the atrium and the ventricle. We subjected astemizole to zebrafish embryos, and applied our apparatus to precisely detect heart arrhythmia (Atrium–Ventricle block) induction. In other words, we provided a simple, economical, and reliable method to measure cardiac rhythm in zebrafish embryos, which is able to evaluate whether cardiac rhythm is affected by chemical compounds.
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Sampurna et al. (2018) studied Cardiac rhythm / Arrhythmia. ImageJ-based method and astemizole exposure was evaluated on Cardiac rhythm and heart arrhythmia (Atrium-Ventricle block) induction. A simple ImageJ-based method using a dissecting microscope and CCD camera successfully measured cardiac rhythm and detected astemizole-induced heart arrhythmia in zebrafish embryos.
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