Glycolysis was more effective than oxidative phosphorylation in preventing ATP-sensitive K+ channels from opening in isolated guinea pig cardiac myocytes.
Does glycolysis preferentially inhibit ATP-sensitive K+ channels compared to oxidative phosphorylation in isolated guinea pig cardiac myocytes?
Glycolysis is more effective than oxidative phosphorylation at inhibiting ATP-sensitive K+ channels in cardiac myocytes, highlighting a localized mechanism for ATP delivery to membrane channels that may be relevant during myocardial ischemia.
In heart, glycolysis may be a preferential source of adenosine triphosphate (ATP) for membrane functions. In this study the patch-clamp technique was used to study potassium channels sensitive to intracellular ATP levels in permeabilized ventricular myocytes. Activation of these K+ channels has been implicated in marked cellular K+ loss leading to electrophysiological abnormalities and arrhythmias during myocardial ischemia. The results showed that glycolysis was more effective than oxidative phosphorylation in preventing ATP-sensitive K+ channels from opening. Experiments in excised inside-out patches suggested that key glycolytic enzymes located in the membrane or adjacent cytoskeleton near the channels may account for their preference for glycolytic ATP.
Weiss et al. (Fri,) conducted a other in Isolated guinea pig cardiac myocytes. Glycolysis vs. Oxidative phosphorylation was evaluated on Opening of ATP-sensitive K+ channels. Glycolysis was more effective than oxidative phosphorylation in preventing ATP-sensitive K+ channels from opening in isolated guinea pig cardiac myocytes.