What question did this study set out to answer?

This research investigates the role of CCAAT/enhancer-binding protein alpha in regulating the transcription of the beta(3-adrenergic receptor gene during adipogenesis.

January 1, 2001Journal of Biological ChemistryOpen Access

CCAAT/Enhancer-binding Protein α Is Required for Transcription of the β3-Adrenergic Receptor Gene during Adipogenesis

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Authors

TDTonya M. DixonKing's College - North Carolina KDKiefer W. DanielZen Bio (United States)SFStephen R. FarmerBoston University

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Implication

Randomized trial demonstrates the role of C/EBP alpha in regulating beta(3)AR expression during adipogenesis, suggesting implications for metabolic health.

Key Points

This research investigates the role of CCAAT/enhancer-binding protein alpha in regulating the transcription of the beta(3-adrenergic receptor gene during adipogenesis.
Isolated and sequenced a 5.13-kilobase pair fragment of the mouse beta(3)AR promoter.
Conducted luciferase reporter gene assays in adipocytes and fibroblasts.
Performed electrophoretic mobility shift analysis to assess protein-DNA interactions.
The beta(3)AR promoter increased luciferase expression 50-fold in adipocytes.
Insertions of both wild-type and mutated C/EBP binding sites were tested, showing a 3-fold increase in reporter expression with the wild-type.
The functional C/EBP binding site at -3306 was confirmed through specific protein-oligonucleotide complex formation.

Cite This Study

Dixon et al. (2001) studied this question.

synapsesocial.com/papers/6a64a9b92426650dfdc96d73 https://doi.org/10.1074/jbc.m008440200

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Implication

Randomized trial demonstrates the role of C/EBP alpha in regulating beta(3)AR expression during adipogenesis, suggesting implications for metabolic health.

CCAAT/Enhancer-binding Protein α Is Required for Transcription of the β3-Adrenergic Receptor Gene during Adipogenesis

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Implication

Key Points

Cite This Study

CCAAT/Enhancer-binding Protein α Is Required for Transcription of the β3-Adrenergic Receptor Gene during Adipogenesis

Discussion

Member takes

Implication

Key Points

Cite This Study

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