Key result
The splint DNA ligation method using modified splint DNAs with 2,6-diaminopurine improved the circularization efficiency of lengthy RNAs compared to conventional enzymatic methods.
Why the study?
Producing large proteins from circular RNAs has been difficult due to the low circularization efficiency of lengthy precursor linear RNAs.
A modified splint DNA ligation method using RNA ligase 1 and DAP-modified splint DNAs significantly improves the efficiency of synthesizing large circular RNAs.
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May advance circRNA lab methods; leaves open clinical translation pending trials.
Kim et al. (2023) studied this question. Splint DNA ligation method using modified splint DNAs vs. Conventional enzymatic method without splint DNAs was evaluated on Circularization efficiency of lengthy RNAs. The splint DNA ligation method using modified splint DNAs with 2,6-diaminopurine improved the circularization efficiency of lengthy RNAs compared to conventional enzymatic methods.
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