Key result
Receptor-mediated stimulation or depolarization results in phosphorylation of myosin light chain by myosin light chain kinase alone, whereas phorbol esters increase phosphorylation by protein kinase C.
Population
Bovine tracheal tissue and cultured tracheal smooth muscle cells
Comparison
Treatment with carbachol, isoproterenol, phorbol… vs Control conditions
Design
Preclinical
Authors
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Questions relevance of in vitro myosin phosphorylation findings to intact smooth muscle; hypothesis-generating for contractile regulation.
In intact smooth muscle, physiological contraction is mediated by myosin light chain kinase phosphorylation, while phorbol esters activate protein kinase C-mediated phosphorylation.
Kamm et al. (1989) studied Smooth muscle physiology. Pharmacological stimulation (carbachol, phorbol esters, etc.) vs. Control conditions was evaluated on Phosphorylation of myosin heavy and light chains. Receptor-mediated stimulation or depolarization results in phosphorylation of myosin light chain by myosin light chain kinase alone, whereas phorbol esters increase phosphorylation by protein kinase C.
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