Key result
In a reconstituted in vitro system of porcine cardiac sarcoplasmic reticulum and mitochondria, calcium increased thapsigargin-sensitive ATP production up to 5-fold with minimal changes in NADH.
Population
Porcine cardiac sarcoplasmic reticulum (SR) and mitochondria in vitro
Design
Preclinical
Authors
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Extends in vitro evidence for calcium regulation of cardiac ATP synthesis; leaves open applicability to intact human cardiomyocytes.
Cytosolic calcium provides balanced activation of SR ATPase and mitochondrial ATP synthesis, which may explain the minimal alteration in energy metabolism intermediates during changes in cardiac workload.
Balaban et al. (2003) studied this question. Calcium (Ca2+) was evaluated on ATP production and NADH concentration. In a reconstituted in vitro system of porcine cardiac sarcoplasmic reticulum and mitochondria, calcium increased thapsigargin-sensitive ATP production up to 5-fold with minimal changes in NADH.
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