CRISPR/Cas system-based biosensors offer a flexible, sensitive, and specific emerging technology for the detection of foodborne pathogenic microorganisms.
CRISPR/Cas system-based biosensors represent a highly flexible, sensitive, and specific emerging technology for the detection of foodborne pathogens.
Foodborne pathogens pose significant risks to food safety. Conventional biochemical detection techniques are facing a series of challenges. In recent years, with the gradual development of CRISPR (clustered regularly interspaced short palindromic repeats) technology, CRISPR/Cas system-based biosensors, a newly emerging technology, have received much attention from researchers because of their supreme flexibility, sensitivity, and specificity. While numerous CRISPR-based biosensors have a broad application in the field of environmental monitoring, food safety, and point-of-care diagnosis, they remain in high demand to summarize recent advances in CRISPR/Cas system-based biosensors for foodborne pathogen detection. In this paper, we briefly classify and discuss the working principles of CRISPR/Cas systems with trans-cleavage activity in applications for the detection of foodborne pathogenic microorganisms. We highlight the current status, the unique feature of each CRISPR system and CRISPR-based biosensing platforms, and the integration of CRISPR-Cas with other techniques, concluding with a discussion of the advantages, disadvantages, and future directions.
Xie et al. (Wed,) conducted a review in Foodborne pathogenic microorganisms. CRISPR/Cas system-based biosensors was evaluated. CRISPR/Cas system-based biosensors offer a flexible, sensitive, and specific emerging technology for the detection of foodborne pathogenic microorganisms.