What question did this study set out to answer?

To examine how tube materials and buffer compositions influence the accuracy of Somatomedin-C quantitation via radioimmunoassay.

May 1, 1982The Journal of Clinical Endocrinology & Metabolism

Pitfalls in the Somatomedin-C Radioimmunoassay1

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Authors

RFRichard W. FurlanettoMedical University of South Carolina

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Implication

Laboratory study demonstrates interference from polystyrene tubes and protamine buffers in somatomedin-C assays, highlighting protocol requirements for accurate clinical radioimmunoassays.

Key Points

To examine how tube materials and buffer compositions influence the accuracy of Somatomedin-C quantitation via radioimmunoassay.
Quantitated Somatomedin-C in serum and plasma samples using radioimmunoassay under different experimental modifications.
Compared assay performance using borosilicate glass tubes versus polystyrene tubes and protamine-free buffers versus protamine-containing buffers.
Quantitative measurement of Somatomedin-C is consistently achieved in serum and plasma samples when using borosilicate tubes and protamine-free buffers.
Polystyrene tubes, protamine-containing buffers, or their combination disrupt accurate Somatomedin-C quantitation by altering the hormone's interaction with its binding protein.

Cite This Study

Richard W. Furlanetto (1982) studied this question.

synapsesocial.com/papers/6a6550a63caefa60660334c3 https://doi.org/10.1210/jcem-54-5-1084

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Implication

Laboratory study demonstrates interference from polystyrene tubes and protamine buffers in somatomedin-C assays, highlighting protocol requirements for accurate clinical radioimmunoassays.

Pitfalls in the Somatomedin-C Radioimmunoassay1

Discussion

Member takes

Implication

Key Points

Cite This Study

Pitfalls in the Somatomedin-C Radioimmunoassay1

Discussion

Member takes

Implication

Key Points

Cite This Study

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