What question did this study set out to answer?

This study aims to critically evaluate the effectiveness of a mouse model of myocardial infarction for therapeutic research.

May 11, 2004Experimental PhysiologyOpen Access

Critical appraisal of the mouse model of myocardial infarction

Q: What is the clinical evidence from this study?

Population: Myocardial infarction (mouse model). Intervention: Occluding suture placement at distinct sites along the left anterior descending coronary artery vs. Sham-operated animals. Primary outcome: Maximum left ventricular pressure (p=<0.01).

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Key result

Middle suture placement in a mouse model of myocardial infarction significantly reduced maximum left ventricular pressure compared to sham-operated animals (55.9 vs 80.8 mmHg, P<0.01).

Population

Mouse model of myocardial infarction

Comparison

Occluding suture placed at one of three distinct… vs Sham-operated animals

Design

Preclinical

Follow-up

7 days

Authors

NDNaomi M. DegabrieleLung Institute UGUta GriesenbachCell and Gene Therapy Catapult KSKaori SatoBoston University

Discussion

Member takes

Implication

High proximal LAD ligation limits mouse MI model utility; leaves open optimal site selection for therapeutic studies.

Key Points

This study aims to critically evaluate the effectiveness of a mouse model of myocardial infarction for therapeutic research.
Mice underwent myocardial infarction with suture placement at high, middle, or low sites along the left anterior descending coronary artery.
Survival rates, haemodynamic measurements, and histopathological evaluations were assessed post-MI.
Power calculations were performed to evaluate the potential usefulness of the Middle Suture model.
In the High group, only 33% of mice survived 7 days post-MI (P < 0.05 compared to other groups).
The Middle group showed significantly reduced haemodynamics compared to sham-operated animals (max left ventricular pressure: 55.9 mmHg vs. 80.8 mmHg, P < 0.01).
Histological analysis revealed larger lesions in the Middle group compared to the Low group (P < 0.05).

Structured PICO

Population

Mice subjected to myocardial infarction via occluding suture at three distinct sites along the left anterior descending coronary artery, followed for 7 days.

Intervention

Occluding suture placed at one of three distinct sites along the left anterior descending coronary artery (High, Middle, or Low)

Comparator

Sham-operated animals

Outcome

Survival, haemodynamic measurements, and histopathologysurrogate

Main Result

Absolute Event Rate: 55.9% vs 80.8%

p-value: p=<0.01

Placing the occluding suture at the middle site of the LAD in mice provides a reliable model of myocardial infarction with measurable haemodynamic and histological changes suitable for therapeutic studies.

Limitations

A certain amount of intragroup variation

Cite This Study

Degabriele et al. (2004) studied Myocardial infarction (mouse model). Occluding suture placement at distinct sites along the left anterior descending coronary artery vs. Sham-operated animals was evaluated on Maximum left ventricular pressure (p=<0.01). Middle suture placement in a mouse model of myocardial infarction significantly reduced maximum left ventricular pressure compared to sham-operated animals (55.9 vs 80.8 mmHg, P<0.01).

synapsesocial.com/papers/6a69f808ba3b67e10e447a40 https://doi.org/10.1113/expphysiol.2004.027276

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Critical appraisal of the mouse model of myocardial infarction

Discussion

Member takes

Implication

Key Points

Structured PICO

Main Result

Limitations

Cite This Study

Critical appraisal of the mouse model of myocardial infarction

Discussion

Member takes

Implication

Key Points

Structured PICO

Main Result

Limitations

Cite This Study

Also Consider

Also Consider