Key result
The velocity of myosin-V immunoadsorbed from brain extracts was similar to purified myosin-V, with motility being most robust in the absence of Ca2+.
Population
Brain myosin-V isolated by immunoadsorption from crude tissue extracts
Comparison
Optical tweezer-based assay using Limulus sperm… vs Purified myosin-V antibody-linked to beads or…
Design
Preclinical
Authors
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Validates native myosin-V assays from extracts; extends purified protein data but leaves open in vivo Ca2+ regulation.
A novel optical tweezer-based assay successfully characterized the mechanochemical properties of myosin-V directly immunoadsorbed from crude tissue extracts.
Wolenski et al. (1995) studied this question. Immunoadsorbed brain myosin-V vs. Purified myosin-V was evaluated on Motility and velocity of myosin-V. The velocity of myosin-V immunoadsorbed from brain extracts was similar to purified myosin-V, with motility being most robust in the absence of Ca2+.
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