Key result
Oxidation of rabbit psoas muscle fibers by 50 mM hydrogen peroxide irreversibly inhibited contractility and enzymatic activity by targeting multiple methionine residues in myosin.
Why the study?
Does hydrogen peroxide treatment alter contractility and myosin structure in permeabilized rabbit skeletal muscle fibers?
Does hydrogen peroxide treatment alter contractility and myosin structure in permeabilized rabbit skeletal muscle fibers?
High concentrations of hydrogen peroxide inhibit skeletal muscle fiber contractility via oxidation of multiple methionine residues in myosin, altering its structural state.
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High H2O2 irreversibly impairs skeletal fiber contractility via myosin Met oxidation; leaves open cardiac relevance and in vivo translation.
Próchniewicz et al. (2007) studied this question. Hydrogen peroxide was evaluated on Muscle contractility (isometric force and shortening velocity) and enzymatic activity. Oxidation of rabbit psoas muscle fibers by 50 mM hydrogen peroxide irreversibly inhibited contractility and enzymatic activity by targeting multiple methionine residues in myosin.
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