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February 12, 2010Critical Care MedicineOpen Access

In vivo endotoxin synchronizes and suppresses clock gene expression in human peripheral blood leukocytes*

Beatrice Haimovich Jacqueline E. Calvano Adrian D. Haimovich Steve E. Calvano Susette M. Coyle Stephen F. Lowry

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Key Points

This study aims to assess the impact of endotoxin on clock gene expression in human peripheral blood leukocytes during different circadian phases.
Healthy volunteers received either endotoxin (2 ng/kg) or saline at 0900 or 2100 hrs.
Blood samples were collected pre- and post-infusion to analyze gene expression.
Clock gene expression was measured using quantitative real-time polymerase chain reaction.
Endotoxin caused a suppression of clock gene expression by 80% to 90% in leukocytes within 3 to 6 hours.
Per1 and Per2 gene expression reached their lowest levels between 13 and 17 hours post-infusion.
Cytokine levels peaked and returned to baseline within 6 hours, while clock gene expression remained suppressed for up to 17 hours.

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Implication

Clinical investigation examines how endotoxin affects clock gene expression in human leukocytes, suggesting implications for disease recovery.

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Cite This Study

Haimovich et al. (2010) studied this question.

synapsesocial.com/papers/6a6a292c2de5377d00e89c5c https://doi.org/10.1097/ccm.0b013e3181cd131c

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Keep reading

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