Key result
Ouabain induced Ca2+ overload in wild-type embryonic heart tubes but had no effect on Na+-Ca2+ exchanger knockout (NCX-/-) heart tubes.
This preclinical study provides direct evidence that the Na+-Ca2+ exchanger is absolutely required for the intracellular calcium-modulating effects of cardiac glycosides.
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NCX required for ouabain inotropy in embryonic myocytes; leaves open relevance to adult human heart failure therapy.
Reuter et al. (2002) studied this question. Ouabain vs. Wild-type heart tubes was evaluated on Ca2+ transients and Ca2+ overload. Ouabain induced Ca2+ overload in wild-type embryonic heart tubes but had no effect on Na+-Ca2+ exchanger knockout (NCX-/-) heart tubes.
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