Key result
A modified procedure for preparing junctional terminal cisternae from frozen rabbit muscle increased yield threefold and allowed isolation of the junctional face membrane for in vitro study.
This study describes a novel methodology for isolating and characterizing the junctional face membrane from the sarcoplasmic reticulum of rabbit skeletal muscle, facilitating future studies on excitation-contraction coupling.
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Supports in vitro studies of excitation-contraction coupling; leaves open translation to cardiac muscle or clinical relevance.
Costello et al. (1986) studied this question. Modified preparation procedure for junctional terminal cisternae was evaluated on Yield and characterization of junctional face membrane. A modified procedure for preparing junctional terminal cisternae from frozen rabbit muscle increased yield threefold and allowed isolation of the junctional face membrane for in vitro study.
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