Key result
Chronic heart failure in rats resulted in significantly higher DNA damage across multiple tissues compared to sham (p < 0.001), with the soleus muscle being the most affected.
Why the study?
Chronic heart failure involves structural and functional cardiac alterations, but few investigations had evaluated oxidative DNA damage in this setting.
Does chronic heart failure induced by myocardial infarction increase DNA damage in cardiac and peripheral tissues in a rat model?
Does chronic heart failure induced by myocardial infarction increase DNA damage in cardiac and peripheral tissues in a rat model?
Chronic heart failure induces significant systemic DNA damage that affects both central and peripheral tissues, with skeletal muscle (soleus) being the most affected.
Answers are grounded in this paper and cite their sources.
Systemic DNA damage occurs in rat HF; leaves open human relevance and therapeutic implications.
BACKGROUND: Chronic heart failure (CHF) is a complex syndrome which comprises structural and functional alterations in the heart in maintaining the adequate blood demand to all tissues. Few investigations sought to evaluate oxidative DNA damage in CHF. OBJECTIVE: To quantify the DNA damage using the comet assay in left ventricle (LV), lungs, diaphragm, gastrocnemius and soleus in rats with CHF. METHODS: Twelve male Wistar rats (300 to 330 g) were selected for the study: Sham (n = 6) and CHF (n = 6). The animals underwent myocardial infarction by the ligation of the left coronary artery. After six weeks, the animals were euthanized. It was performed a cell suspension of the tissues. The comet assay was performed to evaluate single and double strand breaks in DNA. Significance level (p) considered < 0.05. RESULTS: The CHF group showed higher values of left ventricle end-diastolic pressure (LVEDP), pulmonary congestion, cardiac hypertrophy and lower values of maximal positive and negative derivatives of LV pressure, LV systolic pressure (p < 0.05). CHF group showed higher DNA damage (% tail DNA, tail moment and Olive tail moment) compared to Sham (p < 0.001). The tissue with the highest damage was the soleus, compared to LV and gastrocnemius in CHF group (p < 0.05). CONCLUSION: Our results indicates that the CHF affects all tissues, both centrally and peripherically, being more affected in skeletal muscle (soleus) and is positively correlated with LV dysfunction.
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Stefani et al. (2020) studied Chronic heart failure (n=12). Myocardial infarction-induced chronic heart failure vs. Sham was evaluated on DNA damage (% tail DNA, tail moment and Olive tail moment) (p=< 0.001). Chronic heart failure in rats resulted in significantly higher DNA damage across multiple tissues compared to sham (p < 0.001), with the soleus muscle being the most affected.
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