Key result
Ischemic factors leading to cytosolic acidosis (low extracellular pH and lactate) cause irreversible cytosolic Ca2+ overload in endothelial cells, primarily sourced from the endoplasmic reticulum.
Population
Cultured rat coronary endothelial cells
Comparison
Exposure to different components of simulated… vs Normoxic cells at pHo 7.4
Design
Preclinical
Authors
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May implicate acidosis in ischemic endothelial Ca2+ injury; hypothesis-generating for ER-targeted therapies pending in vivo validation.
In coronary endothelial cells, ischemic factors causing cytosolic acidosis (low pH and lactate) are the primary drivers of irreversible calcium overload via endoplasmic reticulum release.
Y Ladilov (2000) studied Simulated ischemia. Simulated ischemia components (glucose deprivation, anoxia, low extracellular pH, lactate) vs. Normoxic cells was evaluated on Cytosolic Ca2+, cytosolic pH, and ATP content. Ischemic factors leading to cytosolic acidosis (low extracellular pH and lactate) cause irreversible cytosolic Ca2+ overload in endothelial cells, primarily sourced from the endoplasmic reticulum.
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