A Novel HRAS Mutation Independently Contributes to Left Ventricular Hypertrophy in a Family with a Known MYH7 Mutation

Figures

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  Fig 1. Pedigree showing proband’s family. Squares and circles symbolize males and females, respectively. Closed symbols denote subject presenting with left ventricular hypertrophy. Slashed symbols indicate deceased subjects with unknown genotype and clinical status. The proband is indicated by an arrow. The asterisk indicates the presence of intellectual disability.

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  Fig 2. Histological analysis of myocardial samples. Comparison of interventricular septum specimens of proband and his sister. No major differences are detectable. (Hematoxylin-eosin stained section, 20X original magnification).

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  Fig 3. Electropherograms of the MYH7 and HRAS mutations in the family. Sanger sequence analysis of family members reveals A) a transversion of nucleotide NM_000257.2: c.2795T>A in MYH7 gene in brother and sister of proband (II.1 and II.2) and B) a transition NM_005343.2:c.202C>T in HRAS gene in proband (II.3) and in his daughter (III.2). The pedigree is also shown.

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  Fig 4. Structural analysis of HRAS protein. A) Schematic representation of HRAS structure, showing functional domains. Multiple regions participating in GTP binding are represented by blue pentagons. The switch I domain (red rectangle) is responsible for GAP interaction as well as some effector interactions, while the switch II domain (red oval) interacts with GEF. The green C-terminal box demarcates the hyper-variable regions and orange arrow demarcates CAAX box. B) Multiple-sequence alignment of Ras proteins among species is represented. The residue in position 68 is highlighted in yellow. C) and D) represent structural comparison of the most predominant conformation of HRAS wild-type and HRAS-Arg68Trp respectively from molecular dynamics simulations. In HRAS wild-type (B) the water molecule between the Arg68 and Gly60 is highlighted. In the mutant protein model (C) water molecule is weakly present.

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  Fig 5. HRAS Arg68Trp mutation promotes GTP loading and activation of downstream signaling events. HEK 293T cells transfected with plasmids encoding the indicated HA-tagged Ras mutants were serum starved for 24 hr prior to lysis and detection of HA-tagged Ras, and endogenous ERK or phosphor ERK. Ras-GTP was isolated using GST-Raf-RBD-immobilized beads as detailed in Methods. Luciferase activity was measured from similarly-treated cells following cotransfection with reporter plasmids. Ras expression and ERK activation were confirmed by western blot of lysates used for luciferase activity (not shown).