Cross-Platform Urine Metabolomics of Experimental Hyperglycemia in Type 2 Diabetes

Mid-stream urine was collected from overweight (body-mass index 25-30) people with type 2 diabetes of less than 5 years duration immediately before and 20-30 min after intravenous administration of a bolus of 50 ml 50% w/v glucose. All subjects had normal serum creatinine concentration, and 4 out of 46 had an elevated urinary albumin/creatinine ratio, indicative of microalbuminuria. Baseline venous glucose ranged from 4.2-14.4 mmol/l and peaked between 17.3 and 24.5 mmol/l within 6 minutes of glucose infusion. Samples were stored on ice for up to 1 hour and then frozen at -70C until analysed.