Acute blood glucose fluctuation induced myocardial apoptosis, enhanced oxidative stress, and increased NF-ĸB activation compared with continually high blood glucose (p < 0.05).
Does acute blood glucose fluctuation induce myocardial apoptosis compared to continually high blood glucose in Wistar rats?
Acute blood glucose fluctuation induces myocardial apoptosis in a rat model, mediated by oxidative stress and NF-ĸB activation.
valor p: p=< 0.05
OBJECTIVE: It was the aim of this study to investigate whether acute blood glucose fluctuation induces myocardial apoptosis and to examine the potential mechanisms. METHODS: Wistar rats were infused intermittently or continually with 50% glucose solution for 48 h. Serum and myocardium were taken to measure the levels of malondialdehyde and glutathione peroxidase. The expression of nuclear factor (NF)-ĸB and apoptosis in myocardial cells was determined with immunohistochemisty and terminal deoxynucleotidyl transferase dUTP nick end labeling, respectively. Expressions of B-cell lymphoma/leukemia-2-associated X protein and B-cell lymphoma/leukemia 2 in myocardium were tested with Western blot analysis. RESULTS: The levels of malondialdehyde and B-cell lymphoma/leukemia-2-associated X protein in the acute blood glucose fluctuation group (AFG) were enhanced, but glutathione peroxidase and B-cell lymphoma/leukemia-2 were reduced compared with levels in the continually high blood glucose group (p < 0.05). The expression of NF-ĸB in the nuclei of myocardial cells in the AFG was significantly higher than that in the continually high blood glucose group (p < 0.05). Apoptotic myocytes were observed in myocardium of the AFG. CONCLUSION: Acute blood glucose fluctuation induces myocardial apoptosis, apparently associated with enhanced oxidative stress and activation of NF-ĸB.
Zhang et al. (Wed,) conducted a other in Myocardial apoptosis and oxidative stress. Intermittent infusion of 50% glucose solution (acute blood glucose fluctuation) vs. Continual infusion of 50% glucose solution (continually high blood glucose) was evaluated on Myocardial apoptosis, oxidative stress markers (malondialdehyde, glutathione peroxidase), and NF-ĸB expression (p=< 0.05). Acute blood glucose fluctuation induced myocardial apoptosis, enhanced oxidative stress, and increased NF-ĸB activation compared with continually high blood glucose (p < 0.05).