Key result
Acute blood glucose fluctuation induced myocardial apoptosis, enhanced oxidative stress, and increased NF-ĸB activation compared with continually high blood glucose (p < 0.05).
Why the study?
Does acute blood glucose fluctuation induce myocardial apoptosis compared to continually high blood glucose in Wistar rats?
Does acute blood glucose fluctuation induce myocardial apoptosis compared to continually high blood glucose in Wistar rats?
Acute blood glucose fluctuation induces myocardial apoptosis in a rat model, mediated by oxidative stress and NF-ĸB activation.
Answers are grounded in this paper and cite their sources.
Acute glucose fluctuations may exacerbate myocardial injury in rats; leaves open human relevance of glycemic variability.
OBJECTIVE: It was the aim of this study to investigate whether acute blood glucose fluctuation induces myocardial apoptosis and to examine the potential mechanisms. METHODS: Wistar rats were infused intermittently or continually with 50% glucose solution for 48 h. Serum and myocardium were taken to measure the levels of malondialdehyde and glutathione peroxidase. The expression of nuclear factor (NF)-ĸB and apoptosis in myocardial cells was determined with immunohistochemisty and terminal deoxynucleotidyl transferase dUTP nick end labeling, respectively. Expressions of B-cell lymphoma/leukemia-2-associated X protein and B-cell lymphoma/leukemia 2 in myocardium were tested with Western blot analysis. RESULTS: The levels of malondialdehyde and B-cell lymphoma/leukemia-2-associated X protein in the acute blood glucose fluctuation group (AFG) were enhanced, but glutathione peroxidase and B-cell lymphoma/leukemia-2 were reduced compared with levels in the continually high blood glucose group (p < 0.05). The expression of NF-ĸB in the nuclei of myocardial cells in the AFG was significantly higher than that in the continually high blood glucose group (p < 0.05). Apoptotic myocytes were observed in myocardium of the AFG. CONCLUSION: Acute blood glucose fluctuation induces myocardial apoptosis, apparently associated with enhanced oxidative stress and activation of NF-ĸB.
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Zhang et al. (2012) studied Myocardial apoptosis and oxidative stress. Intermittent infusion of 50% glucose solution (acute blood glucose fluctuation) vs. Continual infusion of 50% glucose solution (continually high blood glucose) was evaluated on Myocardial apoptosis, oxidative stress markers (malondialdehyde, glutathione peroxidase), and NF-ĸB expression (p=< 0.05). Acute blood glucose fluctuation induced myocardial apoptosis, enhanced oxidative stress, and increased NF-ĸB activation compared with continually high blood glucose (p < 0.05).
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