What is the clinical evidence from this study?

Study design: Other. Intervention: Temperature changes (0° to 40°C). Primary outcome: Calcium ion dependence of rabbit skeletal muscle myosin B and myofibrillar ATPase activity.

What question did this study set out to answer?

This study aims to examine how temperature affects calcium ion binding and myofibrillar ATPase activity in skeletal muscle.

November 1, 1968Journal of Biological ChemistryOpen Access

Calcium Ion-dependent Myofibrillar Adenosine Triphosphatase Activity Correlated with the Contractile Response

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Key result

Temperature changes between 0° and 40°C had no significant effects on calcium ion concentrations, and myosin B ATPase activity showed a constant calcium ion requirement across this range.

Population

Rabbit skeletal muscle myosin B and myofibrils

Design

Preclinical

Authors

RMRichard A. MurphyUniversity of Southern California WHWilhelm HasselbachMax Planck Institute for Medical Research

Discussion

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Implication

EGTA-Ca buffers maintain stable [Ca²⁺] from 0-40°C; leaves open applicability to cardiac myofibrillar assays.

Key Points

This study aims to examine how temperature affects calcium ion binding and myofibrillar ATPase activity in skeletal muscle.
Investigated temperature effects on calcium ion binding and myofibrillar ATPase activity.
Measured absorbance to assess initial ATPase activity rates across 0° to 40° temperatures.
Correlated rates of ATPase activity with syneresis under varying temperatures.
At 20°, the half-maximal calcium ion concentration for myosin B ATPase activity was 7.1 ± 1.0 x 10^-7 M.
Temperature increase reduced calcium ion-dependent ATPase activity, particularly during syneresis.
Initial rapid increase in absorbance correlated with ATPase activity rates at all temperatures.

Structured PICO

Population

Rabbit skeletal muscle myosin B and myofibrils

Exposure

Temperature changes between 0° and 40°

Outcome

Calcium ion binding, myosin B and myofibrillar adenosine triphosphatase activity, and myofibrillar syneresissurrogate

The decrease in calcium ion-dependent ATPase activity in myofibrils following the rapid phase of syneresis appears to be a consequence of the contractile event.

Cite This Study

Murphy et al. (1968) studied this question. Temperature changes (0° to 40°C) was evaluated on Calcium ion dependence of rabbit skeletal muscle myosin B and myofibrillar ATPase activity. Temperature changes between 0° and 40°C had no significant effects on calcium ion concentrations, and myosin B ATPase activity showed a constant calcium ion requirement across this range.

synapsesocial.com/papers/6a6ba5fa47a1bfb288f3385d https://doi.org/10.1016/s0021-9258(18)91917-4

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Calcium Ion-dependent Myofibrillar Adenosine Triphosphatase Activity Correlated with the Contractile Response

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

Calcium Ion-dependent Myofibrillar Adenosine Triphosphatase Activity Correlated with the Contractile Response

Discussion

Member takes

Implication

Key Points

Structured PICO

Cite This Study

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Also Consider