Key result
Short-term dietary nitrate supplementation with beetroot juice significantly increased time to exhaustion during hypoxic exercise compared to placebo (513 vs 490 sec, P < 0.05).
Why the study?
Does short-term dietary nitrate supplementation improve exercise tolerance and alter muscle oxygenation profiles in healthy males during hypoxic exercise?
RCT (n=9)
Does short-term dietary nitrate supplementation improve exercise tolerance and alter muscle oxygenation profiles in healthy males during hypoxic exercise?
Absolute Event Rate: 513% vs 490%
p-value: p=< 0.05
Short-term dietary nitrate supplementation improves hypoxic exercise tolerance, potentially by suppressing deoxygenated hemoglobin increases in active muscles and reducing oxygenation in inactive muscles.
Nitrate may enhance hypoxic tolerance via muscle oxygenation shifts; leaves open benefits for performance or other populations.
Whether dietary nitrate supplementation improves exercise performance or not is still controversial. While redistribution of sufficient oxygen from inactive to active muscles is essential for optimal exercise performance, no study investigated the effects of nitrate supplementation on muscle oxygenation profiles between active and inactive muscles. Nine healthy males performed 25 min of submaximal (heart rate ~140 bpm; EXsub) and incremental cycling (EXmax) until exhaustion under three conditions: (A) normoxia without drink; (B) hypoxia (FiO2 = 13.95%) with placebo (PL); and (c) hypoxia with beetroot juice (BR). PL and BR were provided for 4 days. Oxygenated and deoxygenated hemoglobin (HbO2 and HHb) were measured in vastus lateralis (active) and biceps brachii (inactive) muscles, and the oxygen saturation of skeletal muscle (StO2; HbO2/total Hb) were calculated. During EXsub, BR suppressed the HHb increases in active muscles during the last 5 min of exercise. During EXmax, time to exhaustion with BR (513 ± 24 sec) was significantly longer than with PL (490 ± 39 sec, P < 0.05). In active muscles, BR suppressed the HHb increases at moderate work rates during EXmax compared to PL (P < 0.05). In addition, BR supplementation was associated with greater reductions in HbO2 and StO2 at higher work rates in inactive muscles during EXmax. Collectively, these findings indicate that short-term dietary nitrate supplementation improved hypoxic exercise tolerance, perhaps, due to suppressed increases in HHb in active muscles at moderate work rates. Moreover, nitrate supplementation caused greater reductions in oxygenation in inactive muscle at higher work rates during hypoxic exercise.
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Horiuchi et al. (2017) conducted an RCT in Healthy (n=9). Beetroot juice (dietary nitrate supplementation) vs. Placebo was evaluated on Time to exhaustion during incremental cycling (EXmax) (p=< 0.05). Short-term dietary nitrate supplementation with beetroot juice significantly increased time to exhaustion during hypoxic exercise compared to placebo (513 vs 490 sec, P < 0.05).
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