Key result
Overexpression of Ga7 resulted in abnormal morphogenesis starting at the slug stage, whereas null mutations of Ga7 or Ga8 did not reveal obvious growth or developmental defects.
Population
Dictyostelium cells (JH10 and DH1 auxotrophic mutants)
Comparison
Targeted mutation or overexpression of G alpha 7… vs Wild-type or parental cell lines
Design
Preclinical
Authors
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Novel Gα7/8 cloning expands Dictyostelium G-protein diversity; leaves open their signaling roles in development.
Cloning and characterization of G alpha 7 and G alpha 8 in Dictyostelium reveals unique structural features of G alpha 8 and shows that G alpha 7 overexpression disrupts morphogenesis.
Wu et al. (1994) studied Dictyostelium discoideum development. Targeted mutation and overexpression of Ga7 and Ga8 vs. Wild-type cells was evaluated on Developmental morphology and growth. Overexpression of Ga7 resulted in abnormal morphogenesis starting at the slug stage, whereas null mutations of Ga7 or Ga8 did not reveal obvious growth or developmental defects.
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