Key result
An 125I-vinculin gel overlay technique identified vinculin-binding proteins of Mr 220,000, 130,000 (vinculin), and 42,000 (actin) in fibroblasts and HeLa cells.
Population
Chick embryo fibroblasts, Rous sarcoma virus transformants, HeLa cells, and chicken gizzard
Design
Preclinical
Authors
Loading...
Vinculin overlay assay identifies binding partners in vitro; leaves open their roles in cardiac adhesion and mechanosignaling.
The development of an 125I-vinculin gel overlay technique allows for the identification of specific vinculin-binding proteins, including actin and vinculin itself, advancing the understanding of cellular adhesion complexes.
Joann J. Otto (1983) studied this question. 125I-vinculin gel overlay technique was evaluated on Detection of vinculin-binding proteins. An 125I-vinculin gel overlay technique identified vinculin-binding proteins of Mr 220,000, 130,000 (vinculin), and 42,000 (actin) in fibroblasts and HeLa cells.
Synapse has enriched 3 closely related papers on similar clinical questions. Consider them for comparative context: